abstract：:The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...

journal_title：Biochemistry

authors： Yeagle PL,Martin RB,Pottenger L,Langdon RG

更新日期：1978-07-11 00:00:00

abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...

journal_title：Biochemistry

authors： Oldfield E,Meadows M,Rice D,Jacobs R

更新日期：1978-07-11 00:00:00

abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...

journal_title：Biochemistry

authors： Miles HT,Frazier J

更新日期：1978-07-11 00:00:00

abstract：:Complete assignments of nitrogen-15 resonances of gramicidin S have been made in dimethyl sulfoxide, trifluoroethanol, and in a solvent mixture of dimethyl sulfoxide (50%) and methanol (50%). The assignments are achieved by utilizing the secondary structure of gramicidin S, by comparing the nitrogen-15 spectrum of gra...

journal_title：Biochemistry

authors： Khaled MA,Urry DW,Sugano H,Miyoshi M,Izumiya N

更新日期：1978-06-27 00:00:00

abstract：:GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...

journal_title：Biochemistry

authors： Lefèvre JF,Fuchs RP,Daune MP

更新日期：1978-06-27 00:00:00

abstract：:A soluble extract prepared from cells of an Escherichia coli strain carrying the antibiotic resistance plasmid R6K is capable of carrying out the complete process of R6K DNA replication. DNA synthesis in vitro is dependent on the four deoxyribo- and ribonucleotide triphosphates and is sensitive to rifampin and strepto...

journal_title：Biochemistry

authors： Inuzuka M,Helinski DR

更新日期：1978-06-27 00:00:00

abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

journal_title：Biochemistry

authors： Kretzschmar KM,Mendelson RA,Morales MF

更新日期：1978-06-13 00:00:00

abstract：:Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...

journal_title：Biochemistry

authors： Bayley H,Knowles JR

更新日期：1978-06-13 00:00:00

abstract：:A highly sensitive and stable scanning microcalorimeter is employed in a reinvestigation of the effect of cholesterol on multilamellar suspensions of dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC). Below 20 mol % cholesterol the DPPC mixtures give heat-capacity curves each of which can...

journal_title：Biochemistry

authors： Mabrey S,Mateo PL,Sturtevant JM

更新日期：1978-06-13 00:00:00

abstract：:A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...

journal_title：Biochemistry

authors： Miller JS,Burgess RR

更新日期：1978-05-30 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...

journal_title：Biochemistry

authors： Du Vernay VH Jr,Traugh JA

更新日期：1978-05-30 00:00:00

abstract：:Coupling of bovine carboxypeptidase A with diazotized 5-amino-1H-tetrazole increases esterase activity, decreases peptidase activity slightly, and modifies one tyrosyl residue. Subsequent nitration of the azoenzyme has no further effect on esterase activity, decreases peptidase activity markedly, and modifies a second...

journal_title：Biochemistry

authors： Cueni L,Riordan JF

更新日期：1978-05-16 00:00:00

abstract：:The sex steroid binding protein (SBP) of human pregnancy serum was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5alpha-dihydrotestosterone-17beta-succinyldiaminoethyl-(1,4-butanediol diglycidyl ether)-agarose, and preparative polyacrylamide gel electrophor...

journal_title：Biochemistry

authors： Mickelson KE,Teller DC,Petra PH

更新日期：1978-04-18 00:00:00

abstract：:Although the binding of bivalent metal-ion activators to phosphoglucomutase produces substantial changes in the near ultraviolet spectrum of the enzyme, the extent to which aromatic residues are exposed to the aqueous environment, as assessed by means of solvent perturbation spectroscopy (using D2O), does not appear t...

journal_title：Biochemistry

authors： Ray WJ Jr

更新日期：1978-04-18 00:00:00

abstract：:Rhodospirillum rubrum ribulose bisphosphate carboxylase contains two high affinity binding sites for pyridoxal phosphate and two catalytic sites per dimer. However, pyridoxal phosphate binding at only one site is sufficient for inactivation of both catalytic sites. In the presence of 20 mM bicarbonate, 10 mM magnesium...

journal_title：Biochemistry

authors： Whitman WB,Tabita FR

更新日期：1978-04-04 00:00:00

abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

journal_title：Biochemistry

authors： Johnson ME

更新日期：1978-04-04 00:00:00

abstract：:Cell-free extracts from the HTl clone of cultured Chinese hamster lung cells efficiently promote the incorporation of proline into newly synthesized material, 50% of which is digestible to small peptides by highly purified bacterial collagenase. The synthesis of the these products occurs under optimal protein synthesi...

journal_title：Biochemistry

authors： Haralson MA,Frey KL,Mitchell WM

更新日期：1978-03-07 00:00:00

abstract：:Purple membranes of Halobacterium halobium were studied by differential scanning calorimetry. No transition was detected at temperatures below 70 degrees C. A small endothermic transition was seen at about 80 degrees C and a larger one at 100 degrees C. The larger transition is the irreversible denaturation of bacteri...

journal_title：Biochemistry

authors： Jackson MB,Sturtevant JM

更新日期：1978-03-07 00:00:00

abstract：:Pyrimidine oligonucleotides have been shown to prime the E. coli DNA polymerase I repair reaction, specifically and reproducibly. DNA molecules up to 30 nucleotides long have been obtained from the extension of oligopyrimidine primers, 9 to 11 nucleotides long isolated from the complementary (minus) strand of bacterio...

journal_title：Biochemistry

authors： Kaptein JS,Spencer JH

更新日期：1978-03-07 00:00:00

abstract：:The diversity, abundance, and DNA sequence representation of poly(adenylic acid) containing RNA derived from cells of Achlya ambisexualis cultured in defined and undefined media have been determined. The kinetics of hybridization of polyadenylated RNA with complementary DNA were the same for both culture conditions an...

journal_title：Biochemistry

authors： Rozek CE,Orr WC,Timberlake WE

更新日期：1978-02-21 00:00:00

abstract：:The exopeptidases dipeptidyl aminopeptidases I and IV were used to hydrolyze the N-terminal portion of spinach plastocyanin to dipeptides. The enzymes were used individually as well as in a mixture and the dipeptides were analyzed by combined gas chromatography-mass spectrometry. Data are presented for native plastocy...

journal_title：Biochemistry

authors： Seifert WE Jr,Caprioli RM

更新日期：1978-02-07 00:00:00

abstract：:Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniq...

journal_title：Biochemistry

authors： Brosius J

更新日期：1978-02-07 00:00:00

abstract：:The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...

journal_title：Biochemistry

authors： Savage MJ,Sala-Trepat JM,Bonner J

更新日期：1978-02-07 00:00:00

abstract：:The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...

journal_title：Biochemistry

authors： Witters LA,Avruch J

更新日期：1978-02-07 00:00:00

abstract：:The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...

journal_title：Biochemistry

authors： Dabrowska R,Sherry JM,Aromatorio DK,Hartshorne DJ

更新日期：1978-01-24 00:00:00

abstract：:The Ca2+-dependent, reversible, interaction of cyclic adenosine 3',5'-monophosphate (cAMP) phosphodiesterase with its activator has been used to purify the enzyme by affinity chromatography. Activator-dependent cAMP phosphodiesterase is only a minor component of the proteins specifically adsorbed in the presence of Ca...

journal_title：Biochemistry

authors： Klee CB,Krinks MH

更新日期：1978-01-10 00:00:00

abstract：:Deazaflavins have been found to act as potent catalysts in the photoreduction of flavoproteins in the presence of EDTA and other "photosubstrates". In distinction to the catalysis brought about by normal flavins which involves dark reaction of the photoreduced flavin catalyst, the mechanism of the catalysis by deazafl...

journal_title：Biochemistry

authors： Massey V,Hemmerich P

更新日期：1978-01-10 00:00:00

abstract：:Previous crystallographic studies in this laboratory demonstrated that immunoglobulin light chains with the same amino acid sequence can have at least two and probably three or more conformations, depending on whether the second member of an interacting pair is a light or heavy chain. If a heavy chain is not available...

journal_title：Biochemistry

authors： Firca JR,Ely KR,Kremser P,Westholm FA,Dorrington KJ,Edmundson AB

更新日期：1978-01-10 00:00:00

abstract：:Laser light scattering has been applied to a systematic study of a heterogeneous solution of tubulin at low temperature--conditions under which tubulin assembly into microtubules does not take place. Methods of analyzing laser light scattering results obtained from solutions containing multiple components are discusse...

journal_title：Biochemistry

authors： Gethner JS,Flynn GW,Berne BJ,Gaskin F

更新日期：1977-12-27 00:00:00

abstract：:The diazomethyl ketones of z-Phe-Phe inactivate papain by a stoichiometric reaction at the active-center thiol. Since the reagents are stable in mercaptoethanol, their reaction with papain is judged to be the result of complex formation characteristic of affinity-labeling reagents. The diazomethyl ketones react by a m...

journal_title：Biochemistry

authors： Leary R,Larsen D,Watanabe H,Shaw E

更新日期：1977-12-27 00:00:00

abstract：:When partially purified Ehrlich ascites tumor RNA polymerase II was further purified on a column of phosphocellulose, stimulation of its catalysis of RNA synthesis by stimulatory factor S-II was greatly decreased. This decrease in sensitivity to the stimulatory factor was reversible: the enzyme eluted from phosphocell...

journal_title：Biochemistry

authors： Kuroiwa A,Mizuno D,Natori S

更新日期：1977-12-27 00:00:00

abstract：:We have measured the concentration of mRNAs coding for immunoglobulins, k and lambda type light chains and gamma 1 type heavy chain, in mouse spleen cells activated by bacterial lipopolysaccharide or sheep red blood cells. These mRNAs were quantitated by hybridization to radioactive DNA complementary to highly purifie...

journal_title：Biochemistry

authors： Honjo T,Shimizu A,Tsuda M,Natori S,Katatoka T,Dohmoto C,Mano Y

更新日期：1977-12-27 00:00:00

abstract：:Water deuteron NMR spectra have been studied for the system dipalmitoyllecithin (DPL)-heavy water (D2O) at different compositions and temperatures. From an analysis of the spectra in terms of quadrupole splittings, a phase diagram has been constructed for the temperature range 25-60 degrees C and the composition range...

journal_title：Biochemistry

authors： Ulmius J,Wennerström H,Lindblom G,Arvidson G

更新日期：1977-12-27 00:00:00

abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...

journal_title：Biochemistry

authors： Sparrow JT,Pownall HJ,Hsu FJ,Blumenthal LD,Culwell AR,Gotto AM

更新日期：1977-12-13 00:00:00

abstract：:Human erythrocyte spectrin prepared from fresh blood is a mixture of different association states. Depending on the manner of preparation, the two-chain dimer or the tetramer predominates. These forms are not in rapid thermodynamic equilibrium. The molecular weight of the dimer by sedimentation and diffusion and by li...

journal_title：Biochemistry

authors： Kam Z,Josephs R,Eisenberg H,Gratzer WB

更新日期：1977-12-13 00:00:00

abstract：:Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC 2.6.1.30) with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...

journal_title：Biochemistry

authors： Gilmer PJ,McIntire WS,Kirsch JF

更新日期：1977-11-29 00:00:00

abstract：:14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...

journal_title：Biochemistry

authors： Reisler E,Burke M,Harrington WF

更新日期：1977-11-29 00:00:00

abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...

journal_title：Biochemistry

authors： Bustin M,Simpson RT,Sperling R,Goldblatt D

更新日期：1977-11-29 00:00:00

abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

journal_title：Biochemistry

authors： Robbins AR,Baker RM

更新日期：1977-11-15 00:00:00